Part:BBa_K1456001

Human Tissue Plasminogen Activator (htPA)

• It has already been told that this protein is the most potent and efficient thrombolytic agent popular in clinical practice. It does this by activating plasminogen, a common anticoagulative factor zymogen found in normal bloodstream. By the conversion of plasminogen into plasmin, an enzyme that catalyzes the reaction of the cleavage of fibrin, the main clot component, thrombolysis begins to degrade the clot. (IUBMB Enzyme Nomeclature 1992; MetaCyc 2014)

This activity has been shown synthetically in E.coli and experimentally in vitro. (Pennica et al. 1983; Van de Werf et al. 1984)

• According to the tPA assay results we obtained, we were able to prove that the tPA enzyme could be produced and secreted from the cell successfully hence also showing that tPA is functionally active.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 5
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 119
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 958